Preparation of unfixed undemineralized bone sections: the Bright bone cryostat

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Preparation of unfixed undemineralized bone sections: the Bright bone cryostat.

A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared. The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.

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Enzyme histochemical analysis on cryostat sections of human bone marrow.

A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The...

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The Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Diagnosis of Bone Marrow Metastases

Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...

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The histochemical demonstration of alkaline phosphatase in unfixed frozen sections.

Although, ideally, enzyme histochemistry should be performed on unfixed sections, so as to avoid potential artifacts inherent in chemical fixation, it is usually considered necessary to fix sections so as to stop the 'soluble' enzymes from becoming lost into the incubation medium. However, provided that suitable frozen sections are used, alkaline phosphatase can be demonstrated fully in unfixed...

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Preparation of Sections from Bone Marrow Aspirates and Cellular Exudates Using Soluble Plastic Centrifuge Tubes.

Sections are frequently helpful in the examination of bone marrow aspirates and of the cellular elements of body fluids. The technical difficulties involved have resulted in the elaboration of several time-consuming methods, often needing considerable skill and experience (Berman, 1953; Raman, 1955). During the last few years, the manufacture of chloroform-soluble plastic tubes has made availab...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1972

ISSN: 0021-9746

DOI: 10.1136/jcp.25.1.26